Kyotera district is located in the Central Region of Uganda. It was curved out of Rakai District in 2017 and borders several districts: Maska District to the North, Kalungu District to the Northeast, Lwengo District to the East, Rakai District to the South, Isingiro District to the West, and the Republic of Tanzania to the southwest.
Rakai District borders Lyantonde District to the Northwest, Lwengo District to the North, Masaka District to the Northeast, Kalangala District to the East, the Kagera Region in the Republic of Tanzania to the South, Isingiro District to the Southwest and Kiruhura District to the Northwest.